电离辐射通过转化生长因子-β-介导的上皮-间质叠加来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨宇宙射中央线是否可通过升华酪氨酸-β（TGF-β）-抑制的上皮-间质匹配 (EMT)来促进癌细胞内的肆虐迁移。使用分之一2Gy(60)Coγ中央线照射源自人类器官的6种癌细胞内，记录下来与EMT相关的巨大变化，这最主要分别借助于显微镜技术，胺基酸其内作律，免疫荧光技术，划痕测试和Transwell于大测试来观察并样品细胞内组织形态，EMT标识，肆虐迁移灵活性等。使用酶联免疫吸附律样品这些癌细胞内中TGF-β复合物水平，借助于特别类固醇SB431542来评估TGF-β信号通路在宇宙射中央线EMT中的作用。经过分之一为2Gy照射的癌细胞内中长期存在间叶细胞内的表达，与假照射组相比其上皮标识下降，间叶细胞内标识增加，同时其肆虐移到灵活性增强，TGF-β复合物水平也降低。全面性发现由A549宇宙射中央线诱发的EMT可通过对TGF-β信号抑制发生逆转。这些结果表明TGF-β抑制的EMT在宇宙射中央线诱发增强癌细胞内肆虐移到灵活性中起着关键作用。

校对： lizexiu